Mouse Anti-CD24 Recombinant Antibody (TAB-006LC)
CAT#: TAB-006LC
This product is a recombinant mouse antibody that recognizes CD24 (Cluster of differentiation 24). The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Tested Data
Gene Expression
ELISA
Figure 1 Anti-Human CD24 Recombinant Antibody (TAB-006LC) in ELISA
ELISA analysis of TAB-006LC was performed by coating with Recombinant Human CD24, His tag. Then blocked with BSA, and incubated with Anti-Human CD24 Antibody (TAB-006LC). The HRP-conjugated goat anti-human IgG was used as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-Human CD24 Recombinant Antibody (TAB-006LC) in WB
Western blot analysis of TAB-006LC was performed with Recombinant Human CD24, His tag in non-reduced (lane 1, 0.5 μg) and reduced condition (lane 2, 0.5 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-006LC and HRP Goat Anti-Human IgG was used as a secondary antibody. Chemiluminescent detection was performed.
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Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/45879/1529_A2_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/1725_B2_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong membranous positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_B_5_1_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_A_7_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/78471/168061_A_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (NTERA-2, RPTEC TERT1, RT4, SK-BR-3)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000272398-CD24
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human CD24 (Cluster of differentiation 24)
- Species Reactivity
- Human
- Applications
- FC, FuncS
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
REVIEWS AND Q&AS
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High Binding Affinity
The binding affinity of this antibody is remarkable. It binds to CD24 with high specificity, making it extremely useful for various detection assays.
Great for Western Blots
The Mouse Anti-CD24 Recombinant Antibody (TAB-006LC) performed exceptionally well in Western blots, providing strong signals and confirming the expression of CD24 in our samples.
User-friendly Protocols
The supplier provided comprehensive protocols that were easy to follow. The antibody was straightforward to use and yielded consistent results.
Q&As
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Does this antibody work well with secondary antibodies for detection?
A: Yes, the Mouse Anti-CD24 Recombinant Antibody (TAB-006LC) works well with secondary antibodies conjugated to various detection enzymes or fluorophores for enhanced visualization in different assay formats.
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What is the Mouse Anti-CD24 Recombinant Antibody's format and purity?
A: The antibody is typically supplied in a purified form, with a purity level of over 95% as confirmed by SDS-PAGE. It is available in a liquid format, usually in a buffer containing stabilizers and preservatives.
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Can I use this antibody in an ELISA?
A: Yes, this antibody is suitable for ELISA. It can be used to detect CD24 expression, providing clear and specific labeling of CD24-expressing cells in various tissue types.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-006LC, RRID: AB_3111735)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "CD24"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-007LC | Human Anti-CD24 Recombinant Antibody (TAB-007LC) | FC | Humanized antibody |
| TAB-007LC-S(P) | Human Anti-CD24 Recombinant Antibody; scFv Fragment (TAB-007LC-S(P)) | FC | Humanized scFv |
| TAB-007LC-F(E) | Human Anti-CD24 Recombinant Antibody; Fab Fragment (TAB-007LC-F(E)) | FC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-006LC-S(P) | Mouse Anti-CD24 Recombinant Antibody; scFv Fragment (TAB-006LC-S(P)) | FC | Mouse scFv |
| TAB-006LC-F(E) | Mouse Anti-CD24 Recombinant Antibody; Fab Fragment (TAB-006LC-F(E)) | FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0350MZ | Mouse Anti-CD24 Recombinant Antibody (clone TN4) | ELISA | Mouse IgG1 |
| MRO-1738-CN | Rabbit Anti-CD24 Polyclonal Antibody (MRO-1738-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-XY243 | Mouse Anti-CD24 Recombinant Antibody (clone 4F4E10) | ELISA, WB, FC | Mouse IgG1 |
| VS-0723-WK207 | Human Anti-CD24 Recombinant Antibody (VS-0723-WK207) | ELISA, FC | Chimeric (rabbit/human) IgG1 |
| VS-0723-WK217 | Human Anti-CD24 Recombinant Antibody (VS-0723-WK217) | ELISA, FC | Chimeric (rabbit/human) IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0434-CN-S(P) | Mouse Anti-CD24 Recombinant Antibody (clone E4); scFv Fragment | WB, ELISA, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0433-CN-F(E) | Mouse Anti-CD24 Recombinant Antibody Fab Fragment | WB, ELISA, Neut | Mouse Fab |
| HPAB-0434-CN-F(E) | Mouse Anti-CD24 Recombinant Antibody (clone E4); Fab Fragment | WB, ELISA, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC23 | Rabbit Anti-CD24 Antibody (VS-0924-YC23) - Cancer Stem Cell Marker | IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY27 | CytoStream™ Mouse Anti-CD24 Recombinant Antibody (VS-0225-XY27) | FC | Mouse IgG2a, kappa |
| VS-0225-XY26 | CytoStream™ Mouse Anti-CD24 Recombinant Antibody (VS-0225-XY26) | FC | Mouse IgM, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1145 | Anti-CD24 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1146 | Anti-Mouse CD24 Immunohistochemistry Kit | IHC |
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